Monarch who financed the expedition of Christopher Columbus in 1492, helping to discover a new world. DS, in Spanish, signed “Yo la Reyna (I the Queen),” one page, 8.75 x 12.5, May 30, 1501. Order from the Queen addressed to her Chamberlain, Sancho de Paredes, instructing him to give 251 small pieces of gold ornamentation taken from the sleeves of a shirt belonging to Don Alonso Tellez, to Violante d’Alvyon, the Queen’s servant, for Her Majesty’s use. Signed by Isabella and also signed by Miguel Peres de Almazan, the Queen’s secretary. Bottom portion bears a receipt signed by Ana de Alvyon, bearing the same date, since Violante could not write. In fine condition, with crisp and bold text, expected folds, careful repairs to two “V” shaped cuts on reverse and some mild edge wear. COA John Reznikoff/PSA/DNA and R&R COA.